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. 1999 Sep 6;146(5):917–928. doi: 10.1083/jcb.146.5.917

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JCB9906061.f8a.jpg — Subcellular localization of p193. Indirect immunofluorescence of endogenous p193 (A) and vaults (B) in HeLa cells reveals a punctate cytoplasmic staining pattern with some nuclear speckle staining with affinity-purified p193 antibody but not with the vault mAb (LRP56). By merging the images of A and B, coincident staining is seen as yellow (C), revealing a partial overlap in the cytoplasm and highlighting the nuclear staining by p193. The nucleus is stained with DAPI (D). Preimmune p193 antiserum reveals background staining (E). COS cells transiently expressing VSVG-tagged p193 revealed that the recombinant protein has an expression pattern similar to endogenous p193 (F). PARP is predominantly localized to the nucleus (G).

graphic file with name JCB9906061.f8b.jpg — Subcellular localization of p193. Indirect immunofluorescence of endogenous p193 (A) and vaults (B) in HeLa cells reveals a punctate cytoplasmic staining pattern with some nuclear speckle staining with affinity-purified p193 antibody but not with the vault mAb (LRP56). By merging the images of A and B, coincident staining is seen as yellow (C), revealing a partial overlap in the cytoplasm and highlighting the nuclear staining by p193. The nucleus is stained with DAPI (D). Preimmune p193 antiserum reveals background staining (E). COS cells transiently expressing VSVG-tagged p193 revealed that the recombinant protein has an expression pattern similar to endogenous p193 (F). PARP is predominantly localized to the nucleus (G).