Figure 4.

Peptide competition experiments. CHO lysate was incubated with ¹²⁵I-F*-p23wt in solution in the absence (lane 1) or presence (lanes 2, 3, 4, and 5) of a 10-fold excess of nonradioactive peptides, and irradiated. Coatomer was immunoprecipitated with an anti-γ-COP antibody. Samples were separated on SDS/7.5–16.5% polyacrylamide gradient gels. (A) Immunoblot with antibodies against α-ɛ-COPs. (B) Autoradiogram of A.