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. Author manuscript; available in PMC: 2008 Dec 15.
Published in final edited form as: Immunol Lett. 2007 Oct 16;114(2):86–93. doi: 10.1016/j.imlet.2007.09.008

Figure 1. Characterization of the expression of E7 in cells transfected with the DNA constructs by RT-PCR.

Figure 1

A. Diagrammatic representation of the various DNA constructs used in the current study; pcDNA3-E7, pcDNA3-IL2, pcDNA3-IL2+ pcDNA3-E7 and the fused construct pcDNA3-IL2-E7. B. RT-PCR analysis of E7 expression in cells transfected with the various DNA constructs. RNA were extracted from 293 Db,Kb cells transfected with pcDNA3 (1 ug) (lanes 1,5), pcDNA3-E7 (0.5ug)+pcDNA3 (0.5ug) (lanes 2,6), pcDNA3-E7 (0.5ug) + pcDNA3-IL2(0.5ug) (lanes 3,7), or pcDNA3-IL2-E7(0.5ug) + pcDNA3(0.5ug) (lanes 4,8) using Lipofectamine 2000. In the lanes 1–4, samples were amplified by E7 primers. In the lanes 5–8, samples were amplified by GAPDH primers.