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. Author manuscript; available in PMC: 2008 Dec 15.
Published in final edited form as: Immunol Lett. 2007 Oct 16;114(2):86–93. doi: 10.1016/j.imlet.2007.09.008

Figure 6. Intracellular cytokine staining followed by flow cytometry analysis to determine the activation of E7-specific CD8+ T cells by 293 Db, Kb cells transfected with the various DNA constructs.

Figure 6

293 Db,Kb cells were transfected with the various DNa constructs and incubated with HPV-16 E7 aa49–57 peptide-specific T cells (with a ratio 1:5) at the presence of 1 ml/ml of GolgiPlug (BD Pharmingen) at 37C overnight. Intracellular staining of IFN-g, flow cytometry and data analysis were performed. A. Representative data of intracellular cytokine stain followed by flow cytometry analysis showing the number of E7-specific IFNγ+ CD8+ T cells in the cells transfected with the various DNA constructs.