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. Author manuscript; available in PMC: 2007 Dec 31.
Published in final edited form as: FEBS Lett. 2007 Nov 26;581(29):5727–5731. doi: 10.1016/j.febslet.2007.11.038

Figure 2. AMPK is not required for hypoxic activation of HIF-1.

Figure 2

A. HIF-1α protein levels in AMPKα WT and AMPKα1−/−α2−/− cells exposed to 21% O2 (N)± 1mM DMOG (D) or to 1.5% O2 (H) for 2 hours. Anti-POL II antibody used as loading control.

B. HIF-1α protein levels in AMPKα1−/− and AMPKα2−/− cells exposed to 21% O2 (N)± 1mM DMOG (D) or to 1.5% O2 (H) for 2 hours. Anti-POL II antibody used as loading control.

C. AMPKα WT and AMPKα1−/−α2−/− cells transfected with a HRE-Luciferase reporter gene construct and exposed to 21% O2 ± 1mM DMOG or to 1.5% O2 for 16 hours before being harvested. Firefly luciferase activity was normalized to renilla luciferase activity and data reported as fold induction over 21%O2 AMPKα WT.