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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: Dev Biol. 2007 Sep 22;312(1):245–257. doi: 10.1016/j.ydbio.2007.09.020

Fig. 4.

Fig. 4

Time course with U0126 during tailbud and post-settling stages reveals temporal differences in MEK dependence. (A) Early tailbud (stage 19) treatment with U0126 at 10 μM shows that after hr 4, atrial primordia development is unaffected by the inhibitor. Control DMSO-only treatment has no effect. (B) Post-settling treatment with 40 μM U0126 reveals that, except for first 12-hr period following attachment, treatment during any of the next three 12-hr periods results in a loss of gill slits. Likewise, treatment for either of the 24-hr periods following attachment result in a loss of gill slits. DMSO-treatment during any of these periods does not result in gill slit loss. Numbers above bars indicate number of affected animals over the total observed.