Fig. 3.
Homologous recombination between MG192 and MgPar 8 in the M. genitalium type strain G37 during in vitro passage. A. Alignment of a portion of the MG192 variable region and MgPar 8 in G37-P1 and G37-P35 showing the sequence exchange in the recombination site. Identical sequences are highlighted in the same colour. The number of plasmid clones analysed for each locus is listed in Table 3. All clones analysed for each locus showed homogenous sequence except for the AGT short tandem repeat number variation, with only the most common repeat number shown in this alignment. The sequences of the MG192 and MgPar 8 identified in G37-P35 have been submitted to GenBank under Accession No. EF117280 and EF7289 respectively. B. A model of homologous recombination between MG192 and MgPar 8 in the G37 type strain. Nucleotide positions shown are relative to the full-length MG192 or MgPar 8 of G37-P1, both of which are identical to those of G37T. Regions containing the longest stretch of identical sequences are indicated by the same shading and colour. The regions between dotted vertical lines (corresponding to the sequences shown in A) indicate where sequence exchanges took place, with reciprocal exchange (gene cross-over) shown by two curved arrows and non-reciprocal exchange (gene conversion) shown by one straight arrow.