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. 2007 Oct;66(1):220–236. doi: 10.1111/j.1365-2958.2007.05911.x

Fig. 5.

Fig. 5

MG192 and MgPar sequence variation in M. genitalium ATCC strains TW10-5G and TW48-5G. A. Schematic representation of the MG192 variable region and MgPar 2 in TW10-5G.ATCC compared with those of G37T. Numbers below and above each box refer to the nucleotide positions in the full-length MG192 and MgPar 2 of G37T. Regions containing identical sequences were indicated by the same shading and colour. The regions included in the box with dashed lines indicate where sequence exchanges potentially took place (detailed sequence alignment for this regions is given in Fig. S1). A reciprocal exchange of a 152 bp fragment is indicated by two curved arrows. Another reciprocal exchange of a single base occurred at nt 1044 of MG192 with nt 2118 of MgPar 2. The MgPar 2 sequence in TW10-5G.ATCC was obtained by sequencing of nine plasmid clones, all of which contained the same sequence except for the expected AGT repeat number variation (from 15 to 17 repeats). The sequences of the MG192 and MgPar 2 in TW10-5G.ATCC have been submitted to GenBank under Accession No. EF117281 and EF117290 respectively. B. Schematic diagram of the MG192 variable region and MgPars 8 and 9 in TW48-5G compared with those of G37T. Numbers below and above each box refer to the nucleotide positions in the full-length MG192, MgPar 8 or MgPar 9 of G37T. Regions containing identical sequences were indicated by the same shading and colour. The regions included in the box with dashed lines indicate where sequence exchanges took place (detailed sequence alignment for this regions is given in Fig. S2). A non-reciprocal sequence exchange of a 114 bp fragment was indicated by one straight arrow, and a reciprocal sequence exchange of a 561 bp fragment was indicated by two curved arrows. Both MgPars 8 and 9 sequences in TW48-5G were obtained by sequencing of 10 plasmid clones for each MgPar; all clones for each MgPar contained identical sequences except for the expected AGT repeat number variation (9–11 repeats for MgPar 8 and 8–10 repeats for MgPar 9). The sequences of the MG192 and MgPar 8 in TW48-5G have been submitted to GenBank under Accession No. EF117282 and EF117292 respectively.