Table 4.
Primers used to amplify the M. genitalium MG192 and MgPar sequences.
| Primer name | Sequence (5′→3′) | Target (sequence locationa) |
|---|---|---|
| 5346F | TACCCCAACCAAACCAACTC | MG192 (225850–225869) |
| 227567R | CTTCGCAATTCATTAGGGGAGACA | MG192 (227546–227569) |
| 227529R | GATCTGATCAGTTCTGGAAGGTAAACG | MG192 (227505–227531) |
| MG192Ab | CACTAGCCAATACCTTCCTTGTCAAAGAGG | MG192 (225974–226003) |
| 1F2 | GAATGGCGGTTTTTACCCAG | MgPar 1 (86583–86602) |
| 1R1 | AATTTCACACCAACAATGCC | MgPar 1 (88396–88415) |
| 124F3 | TCAGTTTATTGGTCAAAGAAGTCGC | MgPars 1, 2 and 4 (168272–168296) |
| 2F1 | CAAGGGGTTGTTTCTTTGC | MgPar 2 (166960–166978) |
| 2R1 | GTTCTAGGATCAATTCTTGCAAA | MgPar 2 (168687–168709) |
| 170116R | TACTCACCCGTTCGCCTCTA | MgPar 2 (170095–170114) |
| 3F3 | CCAGCAGTTAAGCACAGTGG | MgPar 3 (174730–174749) |
| 3R4 | CTTTCACTACCTGTTTCACG | MgPar 3 (176120–176139) |
| 4F3 | GGTCAAAGAAGTCGCAAATTTTCTTA | MgPar 4 (214652–214677) |
| 4R1 | ATGCATGCAGTGGCTATGG | MgPar 4 (216074–216092) |
| 5F4 | TGGCGGTTTTTATCAACTAAATAAAAAC | MgPar 5 (230434–230461) |
| 5R2 | GCGGTTTTAATCTTTTGGAG | MgPar 5 (232126–232145) |
| 6F1 | TGCAGCCATTTCTTTGATCC | MgPar 6 (273024–273043) |
| 6R1 | CATCTGGTAACCAAAGACAC | MgPar 6 (273872–273891) |
| 7F2 | TGCTGCGGTGAATTCTAAGG | MgPar 7 (312727–312746) |
| 7F3 | TTAATTAATAAAACCTCGACCC | MgPar 7 (314133–314154) |
| 7R1 | CAGCGCCAAAAAGATTAGGA | MgPar 7 (315817–315836) |
| 7R3 | ATGGATGGTTTGGTCAGCG | MgPar 7 and MG192 (314170–314188) |
| 8F1 | GGTAAGCCAATCCGATATTGAG | MgPar 8 (348804–348825) |
| 8F4 | ATGGTGGTTTTTATCAACTAAATAAAAAAC | MgPar 8 (350254–350283) |
| 8R2 | AATAAAGGTAAAGCCTAGTG | MgPars 5 and 8 (350377–350396) |
| 351964R | CAATCAACTACTTTTCACTTTACACG | MgPar 8 (351940–351965) |
| 9F1 | CTGAAAACTAGCGACTGCATTTAG | MgPar 9 (427858–427881) |
| 9F3 | GGTTTGTTGACATAAACACTCC | MgPar 9 (428248–428269) |
| 9R1 | TTAAGTGCTGCATCCTTCCA | MgPar 9 (430749–430768) |
| 429679R | TTGAATCCTTCACCGGACTATC | MgPar 9 (213693–213714) |
a
Location of the primer on the M. genitalium G37T genome sequence under GenBank Accession No. NC_000908.
b
From reference (Musatovova et al., 2006).