Figure 2.

Distinct steady–state fractionation profiles of different subclasses of nuclear receptor coregulators. T47D or HeLa cell lysate was fractionated on a Superose 6 column and subjected to immunoblot analysis by using coregulator-specific antibodies as indicated. Elution peaks of molecular mass standards are indicated. The relatively sharp elution peaks of SRC-1 and CBP were reproducible. No difference in fractionation pattern was observed between different cell lines.