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. 1998 Sep 29;95(20):11697–11702. doi: 10.1073/pnas.95.20.11697

Figure 4.

Figure 4

SRC-1 and TIF2 can form common complexes in vivo. SRC-1 complexes were collected by incubation with SRC-1 monoclonal antibody and polyclonal antimouse IgG and fractionated by gel filtration. Immunoblotting confirmed the shift of SRC-1 from its elution peak in the absence of preincubation with anti-SRC-1 antibody (−) to earlier fractions in the presence of anti-SRC-1 antibody (+). The relatively broad elution profile of shifted SRC-1 is most likely due to the heterogeneity of immune complexes formed in these fractions.