Fig. 6.

Localization of MreB in the MurG(Ts) strain GS58 grown at the restrictive temperature (42°C) in TY for 2 MDs (A). The MreB helical structure was maintained in the absence of the functional full-length MurG protein. (See for MurG localization in these cells Fig. 1C.) MurG localization in the absence of MreB helix (B). IFM was performed with PA340 (wild type) and PA340-678 (ΔmreBCD) cells grown at 28°C in TY to mid-exponential phase. MurG localizes as multiple foci in the cell envelope and at mid-cell in the wild-type strain (a) and is evenly distributed in the cytoplasm with no clear mid-cell localization in the ΔmreBCD strain, although a packed localization is visible (b). Phase contrast (left) and fluorescence images (right) are shown. The image contains a collection of representative cell. Scale bar equals 1 μm.