Fig. 3.

Protein macroarray analysis of cytokine and chemokine secretion from alveolar type I cell cultures infected with RCoV-P or SDAV. Type I cells were inoculated with RCoV-P, SDAV, or mock inoculated. Media were harvested at (A) 6 h or (B) 24 h after inoculation and incubated with RayBio membrane arrays as directed by the manufacturer. Representative membranes from duplicate experiments are shown. Densitometry data were normalized to internal positive controls on each membrane and graphed as relative units. Open bars = Mock, hatched bars = RCoV-P, black bars = SDAV. Scans of autoradiographs for each membrane are inset with relevant spots boxed with numbers corresponding to cytokines on the graph.