Fig. 4.

CXC chemokines have increased levels of mRNA and protein in alveolar type I cells infected with RCoV-P or SDAV. Type I cells were inoculated with RCoV-P, SDAV, or mock-inoculated. Total cellular RNA was extracted at 6 h and 24 h after inoculation. (A) mRNA expression for CINC-2α, CINC-3, and LIX was evaluated by quantitative RT-PCR as described in Materials and methods. Asterisks (∗) indicate a significant difference compared to mock at the same time point, p < 0.05. (B) Media from type I cell cultures were analyzed by ELISA at 6 h, 24 h, and 48 h after inoculation for the expression of CINC-2, CINC-3, and LIX. Asterisks (∗) indicate significant difference from mock-inoculated cells at the same time point, p < 0.01. All data are average values of duplicate samples from 3 to 6 experiments.