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Expression of EBV latent and lytic genes by RT-PCR. Total RNA was purified from Raji cells induced in the absence or in the presence of 3-ABA and collected at the indicated times. mRNA levels of LMP-1, EBNA2, BZLF1 and BFRF1 were determined by RT-PCR as described in the Methods. Amplification products were separated on a 1.5% agarose gel and stained with ethidium bromide.
