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. Author manuscript; available in PMC: 2008 Jan 1.
Published in final edited form as: Thromb Res. 2006 Dec 22;120(4):597–605. doi: 10.1016/j.thromres.2006.11.007

TABLE 1.

PLA2 activity (nmol/mg protein/min) in the cytosolic or membrane fraction of HCAEC. Cell were pretreated with PX-18 (2 μM), AACOCF3 (5 μM) or BEL (5 μM) for 10 mins prior to stimulation with either thrombin (1 IU/mL, 2 mins) or tryptase (20 ng/mL, 2 mins). PLA2 activity was measured in the presence of 4 mM EGTA using (16:0, [3H] 18:1) plasmenylcholine substrate. Data represent mean ± SEM for three separate experiments.

Cytosolic Fraction Membrane Fraction
Unstimulated Thrombin Tryptase Unstimulated Thrombin Tryptase
No Pretreatment 0.52 ± 0.06 0.47 ± 0.10 0.54 ± 0.08 2.54 ± 0.26 8.79 ± 0.71 6.37 ± 0.82
PX-18 0.45 ± 0.05 0.41 ± 0.07 0.47 ± 0.08 2.39 ± 0.14 8.12 ± 0.29 7.01 ± 0.31
AACOCF3 0.22 ± 0.12 0.28 ± 0.09 0.26±0.10 2.41 ± 0.17 8.44 ± 0.74 6.53 ± 0.74
BEL 0.17 ± 0.03 0.21 ± 0.04 0.22 ± 0.03 1.07 ± 0.13 3.01 ± 0.44 2.79 ± 0.12