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. Author manuscript; available in PMC: 2008 Nov 1.
Published in final edited form as: Dev Cell. 2007 Nov;13(5):646–662. doi: 10.1016/j.devcel.2007.08.011

Fig. 6. Coordination of leading edge dynamics is lost in cells with increased active cofilin.

Fig. 6

(A) Kymographs of protrusion and retraction dynamics of the leading edge of a migrating PtK1 control cell, or a cell expressing RacQL alone, RacQL + LIMK DN, RacQL + CFL SA, or CFL SA alone. Edge displacements are encoded with warm color (red) for protrusion and cold color (blue) for retraction. t = 30 sec; d = 5 μm.

(B) Average velocity of a 40 μm leading edge section over 10 min (blue bars, ± SEM). *, P < 0.05 compared to zero average velocity. Average of the absolute velocity of a 40 μm leading edge section over 10 min (purple bars, ± SEM). **, P < 0.05 vs. control cells. Levels of active cofilin as percentage of the level in cells expressing LIMK TE (red line).

(C) Protrusion efficiency. Black *, P < 0.05 compared to a protrusion efficiency of one. n = 4 to 11 cells for each condition.