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. Author manuscript; available in PMC: 2008 Jun 1.
Published in final edited form as: Development. 2007 Dec;134(23):4297–4306. doi: 10.1242/dev.009282

Fig. 5. aPKC functions upstream of PAR1 to specify ectodermal cell fates.

Fig. 5

Embryos were injected with RNAs or MO as described in Fig. 1. Ciliated cells were detected at stages 14-16 by in situ hybridization with the α-tubulin probe. (A-C) T560A reverses the inhibitory effect of aPKC-CAAX on ciliated cell differentiation. Two sides of the same embryo are shown in B and C. (D-F) PAR1B MO (F) suppresses aPKC-N-mediated expansion of ciliated cells. The injected and uninjected sides of the same embryo are shown in D and E, respectively. (G-J) Quantification of the data shown in A-C (G,H) and D-F (I,J). Numbers of embryos per group are shown above bars. (G,I) Frequencies of embryos showing visible phenotypic changes. (H,J) Mean numbers of α-tubulin-positive cells per section±s.d. are shown. Sections of at least three representative embryos per group were analyzed.