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VEGF-B186 is proteolytically processed. Metabolically labeled VEGF-BkEx1–5, VEGF-B167, and VEGF-B186 were immunoprecipitated (IP) with the affinity-purified N-terminal VEGF-B antibody or bound to VEGFR-1-Ig. The bound ligands were analyzed by SDS/PAGE in nonreducing conditions.
