Figure 4.

Recombinant hVEGF-B₁₈₆ increases the expression and activity of uPA and PAI-1 in BME cells. (A) Replicate filters, containing 5 μg per lane of total cellular RNA prepared from confluent monolayers of BME cells incubated in the presence of 50 ng/ml hVEGF-B₁₈₆ for the indicated times, were hybridized with ³²P-labeled cRNA probes as described in the text. RNA integrity and uniformity of loading were determined by staining the filters with methylene blue after transfer and cross-linking (Bottom); 28S and 18S ribosomal RNAs are shown. (B) Cell extracts prepared from BME cells, incubated in the presence of hVEGF-B₁₈₆, VEGF, or bFGF at the indicated concentrations for 15 hr, were subjected to zymography (Upper) and reverse zymography (Lower) as described in the text.