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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: Dev Biol. 2007 Oct 2;312(1):90–102. doi: 10.1016/j.ydbio.2007.09.039

Figure 4.

Figure 4

Inhibition of the maternal Wnt pathway by DN XTcf-3 inhibits wild-type expression of organizer genes only in the injected cells. 32 cell embryos were injected in the dorsal cells of the indicated tiers with 1 ng LacZ RNA with or without 100 pg DN XTcf-3 RNA. Double in situ hybridization was performed at stage 9 for LacZ (Fast red) and the indicated genes (BM Purple). The drawing (top) shows the blastomere nomenclature of a 32 cell stage embryo (Nakamura and Kishiyama, 1971). All views are dorsal. A-L. Inhibition of organizer gene expression is restricted to the injected area in the marginal tiers B and C (control and DN Xtcf3 RNA injections, Sia 35 and 22 embryos, gsc 20 and 25 embryos, chordin 22 and 32 embryos, respectively). A, C, E, G, I, K are control injections with LacZ RNA only. M-P. D1 injections of LacZ RNA alone (M) or together with DN Xtcf-3 RNA (20 embryos) do not interfere with the expression pattern of chordin in marginal cellls. P is a sagital section of the embryo in O, showing the internal distribution of injected RNA. Bars in A and I indicate 0.2 mm.