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. 1998 Sep 29;95(20):11715–11720. doi: 10.1073/pnas.95.20.11715

Figure 4.

Figure 4

(A) Northern blot analysis of RNA isolated from wild-type Hep3B cells during hypoxia (1.5% O2/5% CO2/93.5% N2) or normoxic CoCl2 in the presence of DPI (3 μM), myxothiazol (100 ng/ml), rotenone (3 μg/ml), ebselen (25 μM), or PDTC (100 μM) for 16 hr. (B) HIF-1 DNA binding in nuclear extracts from wild-type cells during hypoxia (1.5% O2/5% CO2/93.5% N2) or normoxic CoCl2 in the presence of DPI (3 μM), myxothiazol (100 ng/ml), rotenone (3 μg/ml), ebselen (25 μM), or PDTC (100 μM) for 4 hr. (C) Northern analysis of RNA from ρ0-Hep3B cells during CoCl2 (100 μM) in the presence of ebselen (25 μM) or PDTC (100 μM) for 16 hr. (D) HIF-1 DNA binding in nuclear extracts from wild-type or ρ0 cells during CoCl2 in the presence of ebselen (25 μM) or PDTC (100 μM) for 4 hr. C, constitutive; NS, nonspecific.