Figure 3.

Morphology of the svl mutants. (A) Yeast cells from wild-type, SVL, or from svl2, svl7, and svl8 cultures were double-stained with CDCFDA and FM4–64 at 25°C, as indicated. Images of the cells were captured with epifluorescence microscopy separately by using a fluorescein isothiocyanate (FITC) or Texas red filter set, and DIC optics, as indicated. The overlay represents a digital blending of the two separate fluorescence captures. The inset in the svl2 panel represents typical vacuole morphology in vps17Δ cells. [Bar = 5 μm (Inset = 2.5 μm).] (B) SVL and svl 8 strains were grown to early-log phase. Cells were stained with BODIPY-PC and BODIPY-PE, and examined by epifluorescence microscopy, as indicated. (Bar = 5 μm.)