Figure 5.

Flow cytometry of three representative svl mutants. Yeast cells from wild-type, SVL, or the svl12, svl2, and svl3 mutants were double-stained with CDCFDA and FM4–64 after a 60-min preincubation at 38°C, as indicated. The cells were then subjected to flow cytometry using the same conditions as Fig. 2. The white and dark gray areas represent the most and least events, respectively. Representative cells from each sample are shown with digital blending of the two separate fluorescein isothiocyanate and Texas Red filter sets plus DIC optics. The bottom panel is an overlay representing >75% of the population from each strain. (Bar = 5 μm.)