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. 2007 Mar 22;581(Pt 3):941–960. doi: 10.1113/jphysiol.2007.128454

Figure 2.

Figure 2

Localization of Kv2.1 and Kv2.2 subunits A–C, layer V of somatosensory cortex (P35 rat). Scale bar in B refers to A–C. A, confocal image of single 1 μm section with fluorescent staining for monoclonal anti-Kv2.1 (Neuromab: green). Kv2.1 is patchy and distributed on somata and proximal apical dendrites (mostly < 50 μm from soma) of all pyramidal cells. B, polyclonal anti-Kv2.2 (Phosphosolutions: red) Note Kv2.2 is largely restricted to large dendritic shafts. Arrows point out the same dendrites indicated by arrows in C. C, superimposed Kv2.1 and Kv2.2. In a few cases, Kv2.2-positive (red) dendrites are in continuity with Kv2.1-positive (green) dendrites (e.g. arrows). D, layer 3 of somatosensory cortex (different P35 animal). Confocal image of single 1 μm section with superimposed fluorescent staining for monoclonal anti-Kv2.1 (green) and polyclonal anti-Kv2.2 (red). Kv2.2 stains some dendrites, which are en passant from deeper layers (we could not match layer 3 pyramidal cell somata with anti-Kv2.2-stained dendrites). Scale bar = 20 μm. E, EM image of Kv2.2 localization in dendritic spine (between arrows). F, at the EM level, much of the Kv2.2 staining was within the cytoplasm of pyramidal cell apical dendrites. The staining appeared vesicular (e.g. arrows).