Figure 2.

Furosemide, bicuculline, gabazine and Zn²⁺ inhibit the spontaneous current arising from α₆β₂δ receptors A, furosemide blocked the spontaneous activity of α₆β₂δ receptors. Current traces and the concentration–response relationship for furosemide-dependent inhibition of the spontaneous current arising from an oocyte with a high level of expression of α₆β₂δ receptors. The dotted line indicates the zero-current level; the shaded area represents the spontaneous activity. The thick lines below the current traces represent the duration of antagonist application. B, bicuculline and gabazine inhibited the spontaneous activity of α₆β₂δ receptors. Current traces representing bicuculline (5 μm) and gabazine (5 μm) inhibitory action on the spontaneous activity. C, the representative current traces and concentration–response relationship for Zn²⁺ block of the spontaneous current arising from α₆β₂δ receptors.