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. Author manuscript; available in PMC: 2008 Apr 30.
Published in final edited form as: Immunity. 2007 Nov;27(5):751–762. doi: 10.1016/j.immuni.2007.08.020

Figure 4. NKT cell development in competition chimeras.

Figure 4

A. 1:1 mixtures of WT + SLAM-/-, WT + Ly108-/-, WT + CD48-/-, 2B4-/-+ Ly108-/-, CD48-/-+ Ly108-/- bone marrow were injected into lethally irradiated Jα18-/- hosts and chimeras were analyzed at 6-8 weeks. Absolute NKT cell numbers were calculated for each CD45 allele-marked compartment, then divided by the number of lymphocytes in the corresponding CD45 allele-marked fraction and multiplied by the total number of lymphocytes to adjust for differences between the ratio of reconstitution by the two bone marrows which ranged between 0.3 and 0.7. *, ** and *** denote statistical significance (p<0.05, p<0.01 and p<0.001 respectively) using a t test for paired comparisons (2 experiments are pooled as they showed similar results (ANOVA)). B. FACS analysis of MACS-enriched CD1d-αGalCer tetramer+ CD24high and CD24low cells originating from the wt and mutant (CD45 allele-marked) bone marrows. The chimeric distribution of the tetramer-negative thymocytes is also shown (bottom dot plots).