Fig. 1.

Dose-dependent production of NO (A) and TNF-α (B) in RAW 264 cells by LPS treatment. Cells were incubated with LPS (10, 100, or 1000 ng/ml) for 24 h (A) or 12 h (B) at 37°C under a 5% CO₂ and 95% air atmosphere. The levels of NO and TNF-α were measured as described in Materials and Methods. Data points represent the means ± SE (n = 3). ^ap<0.01 vs 0 ng/ml, ^bp<0.01 vs 10 ng/ml, ^cp<0.01 vs 100 ng/ml in (A). ^ap<0.01 vs 0 ng/ml, ^bp<0.05 vs 10 ng/ml, ^cp<0.01 vs 10 ng/ml, ^dp<0.01 vs 100 ng/ml in (B).