Fig. 7.

Effects of GGA on binding of LPS to the cell surface in RAW 264 cells. Cells were incubated with 80 µM GGA for 2 h at 37°C and for further 30 min after addition of LPS (1 µg/ml). Cells were washed, and stained with anti-LPS antibody, followed by goat anti-mouse IgG-FITC. The LPS fluorescence resulting from LPS binding to cells was measured by using Epics cytofluorometer. Representative data from three separate experiments are shown.