Figure 4.

ARFGAP3 colocalizes with coatomer. Methanol–acetone fixed NRK cells were labelled with affinity-purified ARFGAP3 antibodies and mAbs against β-COP, p115, GM130 or γ-adaptin (see Figure 3 for other details). The individual channels are shown in monochrome; the composite overlay is shown to the right (green channel, ARFGAP3; red channel, β-COP, p115, GM130 and γAP, respectively). Bar = 10 μm.