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Sit1-dependent use of FOB-NBD by the cells.Wild-type (WT) or sit1Δ cells were plated on YPD-agar medium supplemented with 1 mm BPS (to prevent reductive iron uptake). Sterile filter paper disks containing siderophores (5 μL of 10 μm CG, FOB or FOB-NBD) were placed on the surface of the agar. Plates were incubated for 2 days at 30°C and photographed. Growing cells appeared as a white halo around the filter papers.
