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. 2005 Oct 24;171(2):255–265. doi: 10.1083/jcb.200506194

Figure 5.

Figure 5.

GαsGFP fluorescence in Gpr3 − / − preantral follicle-enclosed oocytes that had been coinjected with GαsGFP RNA and Gpr3 RNA. Oocytes were injected with 15 pg GαsGFP RNA and the indicated amounts of Gpr3 or a control RNA encoding β-globin, and the follicles were cultured overnight before imaging. (A–C) 7 pg β-globin (A), Gpr3 (B), and 7 fg Gpr3 (C) RNA. The confocal microscope settings and bars were the same for all images. (D) Plasma membrane-to-cytoplasm GαsGFP fluorescence ratios for Gpr3 −/− oocytes that were injected with the indicated RNAs. Mean ± SEM (error bars; n = number of oocytes). Data are from three mice. For the images of oocytes that had been injected with 7 pg Gpr3 RNA (n = 10), no plasma membrane fluorescence could be identified, so no ratio was calculated. Ratios for oocytes that were injected with 7 pg β-globin RNA (7.4 ± 0.5) or 7 fg Gpr3 RNA (2.6 ± 0.2) were significantly different (t test, P < 0.0001). The ratio for Gpr3 −/− oocytes + 7 fg Gpr3 RNA was not different from that for Gpr3 +/+ oocytes (Fig. 4 E; P = 0.14), and the ratio for Gpr3 −/− oocytes + 7 pg β-globin RNA was not different from that for Gpr3 −/− oocytes (Fig. 4 E; P = 0.69).