Figure 5.

Complete maturation of pre-GFP and pre-23K is totally dependent on an intact RR motif. Pre-GFP, pre-GFPΔTPP, pre–KK-GFP, pre–KK-GFPΔTPP, and pre–KK-23K were expressed in protoplasts, followed by pulse and chase treatments as described in Fig. 4. The chloroplasts were isolated and samples prepared of chloroplasts (C), protease-treated chloroplasts (C+), stroma (S), thylakoids (T), and thermolysin-treated thylakoids (T+) as for chloroplast import experiments in Fig. 2. Samples were immunoprecipitated using antibodies to GFP (A) or 23K (B).