Figure 4.

Effect of SSH1L, LIMK1, or cofilin siRNA on SDF-1α–induced T cell chemotaxis and chemokinesis. Jurkat cells were transfected with siRNA plasmids for GFP (control), SSH1L, LIMK1, cofilin, or empty vector (−) as indicated. Chemotactic responses toward 5 nM SDF-1α and chemokinetic responses in the presence of 5 nM SDF-1α were determined in the Transwell culture chambers, as described in Materials and methods. The data are expressed as the means ± SEM (error bars) of three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.005, compared with cells transfected with the empty vector.