Figure 1.

Comparison of nucleotide and derived amino acid sequences of CMP-Neu5Ac hydroxylase cDNAs from mouse, chimpanzee, and human. (A) Comparison of nucleotide sequences. Sequencing of multiple full-length cDNA clones was carried out as described. The ClustalW program (http://www2.ebi.ac.uk/clustalw/) was used to align the sequences. The 92-bp gap in the human cDNA and differences at the 3′ end are indicated with dotted lines. The start and stop codons of the primary ORF are underlined. (B) Comparison of the ORFs of the cDNAs. The putative Reiske iron-sulfur binding region of the mouse and chimpanzee hydroxylase are underlined.