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. 1998 Sep 29;95(20):11751–11756. doi: 10.1073/pnas.95.20.11751

Figure 4.

Figure 4

Chromosomal localization of the human CMP-sialic acid hydroxylase gene. (A) Human/rodent somatic-cell hybrid mapping. Ethidium bromide-stained agarose gel analyzing the products of PCR amplification of samples of the NIGMS human/rodent somatic-cell hybrid mapping panel 2. Lanes labeled 1–22, X, and Y reflect DNA from a chromosome 6 hybrid clone containing the designated human chromosomes. Hamster (Ha), mouse (Mo), and human (Hu) are total genomic DNA from those species used to create the hybrid panel. A negative control (B) is shown as well as 100-bp marker lanes with the 300- and 400-bp bands indicated. (B) FISH mapping. Metaphase FISH localization of genomic BAC clone 443N17 containing the CMP-sialic acid hydroxylase gene to the distal short arm of human chromosome 6. Detection of digoxigenin-labeled probes was achieved with anti-digoxigenin antibodies conjugated to rhodamine (giving the red signal), followed by 4′,6-diamidino-2′-phenylindole dihydrochloride (DAPI) counterstaining of the chromosomes and viewing under an epifluorescence microscope with a triple band pass filter.