Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Oct 24;171(2):383–392. doi: 10.1083/jcb.200503017

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — F-actin–independent integrin clustering and talin recruitment. Epifluorescence of fixed B16F1 and CS-1 cells exhibiting de novo formed β3 integrin clusters. Integrin fluorescence (A and B) and phalloidin stained F-actin (A' and B') in 0.5 mM of Mn²⁺-treated cells (20 min; A and A') or 10 μg/ml of cD/Mn²⁺-treated B16F1 cells (25 min of cD, followed by 20 min of cD/Mn²⁺; B and B'). Immunohistochemical analysis of focal adhesion adaptor proteins recruited to Mn²⁺-induced clusters of β3-EGFP integrins in B16F1 cells (C–G) and nontagged β3 integrins in CS-1 cells (H and I). Pairs of images show the distribution of the EGFP integrin fluorescence (C–G) or anti-β3 staining (H and I) and the respective immunohistochemical localization of talin (C' and H'), vinculin (D' and I'), paxillin (E'), phosphotyrosine (F'), and FAK (G'). Corresponding magnified views of the boxed areas in A–H and A'–H' are shown below each image pair (a–h and a'–h'). Bar, 25 μm.