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. 2005 Oct 10;171(1):121–131. doi: 10.1083/jcb.200505047

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Copyright © 2005, The Rockefeller University Press

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Figure 5. — Membrane structure at PBL activation sites. Laurdan-labeled PBLs were conjugated with beads coated with α-CD3 mAb (A), a combination of α-CD3 and CD28 mAb (B), or CD28 mAb alone (C) and were incubated for 0–60 min at 37°C. Imaging and GP quantification at the contact site were performed as described for Fig. 1. (A) All datasets are significantly different to each other (P < 0.001) with the exception of 23 and 60 min, for which P > 0.05. (B) The mean of 0-min incubation is significantly different from 7, 23, and 60 min (P < 0.001), and differences between 23 and 60 min were also significant (P < 0.05); all other sets of data were not significantly different. (C) Only the means of 0 min is significantly different from 7, 23, and 60 min (P < 0.05). Means (indicated by horizontal lines) and SDs are given in Table I.