Table I. GP values at bead–cell or cell–cell contact sites.
| JCaM2 cells | WT LAT | All Y-F LAT | JCaM2 |
|---|---|---|---|
| α-CD3 mAb 0 min 3 min 7 min |
0.240 ± 0.095 (n = 50) 0.336 ± 0.091 (n = 64) 0.442 ± 0.085 (n = 71)a,d,g,h,n |
0.345 ± 0.082 (n = 49)b,f,l,o | 0.247 ± 0.097 (n = 43)j |
| TfR mAb 7 min |
0.227 ± 0.106 (n = 60)a,k | 0.222 ± 0.111 (n = 63)b,l | 0.233 ± 0.063 (n = 53)m |
| α-CD3 mAb PP2 (7 min) PP3 (7 min) |
0.289 ± 0.104 (n = 35)c,d
0.419 ± 0.053 (n = 34)c |
0.265 ± 0.056 (n = 30)e,f
0.322 ± 0.056 (n = 30)e |
0.265 ± 0.087 (n = 34) 0.242 ± 0.095 (n = 32) |
| 4 mM mβCD α-CD3 mAb (7 min) TfR mAb (7 min) 10 mM mβCD α-CD3 mAb (7 min) TfR mAb (7 min) |
0.347 ± 0.069 (n = 41)g
0.237 ± 0.081 (n = 40) 0.229 ± 0.112 (n = 61)h 0.139 ± 0.078 (n = 41)k |
0.365 ± 0.095 (n = 26) 0.215 ± 0.084 (n = 41) 0.194 ± 0.075 (n = 36)i 0.138 ± 0.096 (n = 44)l |
0.249 ± 0.082 (n = 29) 0.196 ± 0.083 (n = 41) 0.172 ± 0.095 (n = 37)j 0.136 ± 0.080 (n = 39)m |
| α-CD3 mAb (7 min) + 3 min latrunculin TfR mAb (7min) + 3 min latrunculin |
0.252 ± 0.102 (n = 58)n
0.257 ± 0.076 (n = 38) |
0.248 ± 0.084 (n = 52)o
0.347 ± 0.072 (n = 32) |
0.237 ± 0.135 (n = 43) 0.256 ± 0.092 (n = 37) |
| PBL | CD3 mAb | CD3 + CD28 mAb | CD28 mAb |
| 0 min 7 min 23 min 60 min |
0.219 ± 0.068 (n = 43) 0.413 ± 0.06 (n = 44)a,b 0.313 ± 0.074 (n = 46)c,d 0.296 ± 0.095 (n = 45) |
0.194 ± 0.075 (n = 48) 0.343 ± 0.060 (n = 44)a,e 0.354 ± 0.083 (n = 44)c,f 0.312 ± 0.070 (n = 42)g |
0.190 ± 0.076 (n = 40) 0.272 ± 0.086 (n = 44)b,e 0.275 ± 0.089 (n = 46)d,f 0.248 ± 0.097 (n = 42)g |
| Naive T cell: APC | Without antigen | With antigen | |
| 0 min 3 min 7 min 23 min |
0.205 ± 0.099 (n = 105) 0.276 ± 0.109 (n = 95)a 0.241 ± 0.079 (n = 86)b 0.187 ± 0.083 (n = 103)c |
0.227 ± 0.093 (n = 107) 0.370 ± 0.082 (n = 93)a,d,e 0.422 ± 0.071 (n = 83)b 0.300 ± 0.100 (n = 92)c |
|
| 7 min PP2 PP3 + 3 min latrunculin |
0.235 ± 0.114 (n = 51)d
0.402 ± 0.061 (n = 50) 0.206 ± 0.082 (n = 53)e |
Means ± SDs are given for JCaM2 cells, PBLs, and synapses between naive T cells and DCs. For comparisons between WT LAT, All Y-F LAT, and JCaM2 cells, see Figs. 2–4. Superscripts indicate pairs of GP means, which were significantly different with P < 0.001 in JCaM2 cells (a–o), PBLs (e–g), and in naive T cells (a–e) and with P < 0.05 in PBLs (a–d).