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. 2005 Nov 7;171(3):493–503. doi: 10.1083/jcb.200507069

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Copyright © 2005, The Rockefeller University Press

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Figure 4. — Fusogenic exosomes are associated with MPMV. (A) MPMV Env, Gag, and exosomal CD9, CD81, and MHCI were detected by immunoblotting in P70 (see Materials and methods). (B) CD81 is detected on the surface and p27^Ca Gag of MPMV inside of exosomes by immuno-EM (see Materials and methods). The inset is an enlargement of the particle indicated by the arrow. (C) MPMV Env and p27 capsid are associated with exosomal markers CD81 and -63. P70 preparation was incubated with or without biotinylated anti-human CD81 antibody and fractionated in a capture assay using magnetic streptavidin microbeads (see Materials and methods). The unbound fraction (FT), the final wash, and the bound fraction (eluate) were analyzed by immunoblotting. Antibodies to CD63 and -81 were used together for convenience because both identify distinct polypeptides. The experiments in A and B were done multiple times and the experiment in C twice.