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. 2005 Nov 7;171(3):493–503. doi: 10.1083/jcb.200507069

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Copyright © 2005, The Rockefeller University Press

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Figure 7. — Expression of E1A or suppression of p53 is sufficient for proliferation of hybrids induced by MPMV ^E . (A) Detroit 551 cells resistant to puromycin (D0^P), hygromycin (D0^H), transduced with E1A and resistant to hygromycin (DE^H), or transduced with p53 R175H and resistant to hygromycin (Dp53R175H^H) were cultured for 16 h in combinations as indicated with or without MPMV^E and then for an additional 20 d in fresh medium containing hygromycin and puromycin. The cells were then visualized by staining with crystal violet. (B) Cell colonies from various fields of the bottom right plate in A. Note the diversity in cell morphology among the fields. The data are from one out of three experiments, all of which produced similar results. Bars, 200 μm.