Figure 7.

Rho activity upon adhesion to LN5. (A) Wt and Tiam1−/− keratinocytes were seeded on LN5-coated coverslips. After 10, 30, and 60 min, cells were stained with phalloidin. Bar, 20 μM. (B) Growth factor–starved keratinocytes were seeded on a LN5 matrix. After 0, 5, and 30 min, cells were lysed and Rho activity was determined. A representative experiment is shown and quantified in the histogram, which represents the Rho activation (relative to the total Rho levels) in WT and Tiam1−/− cells. (C) Growth factor–starved keratinocytes were seeded on a LN5 matrix as described in B. After 0, 1, and 3 h, cells were lysed and Rho activity was determined and quantified as described in B.