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. 2005 Dec 5;171(5):799–810. doi: 10.1083/jcb.200506083

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Copyright © 2005, The Rockefeller University Press

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Figure 1. — Y2H analysis of the interaction between either the nesprin-related Y2H sequence or the first pair of FNIII domains of β4 with several different ABDs. Interactions were scored + when the plating efficiencies on selective SC-LTHA plates were >30% of those on nonselective SC-LT plates at 5 d of growth. Interactions were scored − when no colonies were detected at 10 d of growth. The percent homology of the other ABDs to the plectin ABD is shown in the column on the left. The results for the interactions of the first pair of FNIII domains with the ABDs listed have been reported previously (Litjens et al., 2003).