Figure 3.

Immunofluorescence localization of tricellulin. (A) Immunofluorescence staining of frozen sections of mouse small intestine, stomach, and kidney doubly with anti-tricellulin pAb (red) and anti-occludin mAb (green). Individual renal tubules in the kidney are represented as dotted lines. Tricellulin was predominantly concentrated at the tricellular contacts, where three occludin-positive bTJs converged. There are also weaker signals at bTJs between two adjacent cells, especially in the small intestine. Bar, 20 μm. (B) Immunostaining of mouse Eph4 cells. Tricellulin (red) was concentrated at each tricellular contact, whereas occludin (green) was distributed along bTJs. The right bottom panel shows vertical sectional images generated from confocal microscopic images. Arrow and arrowheads represent bTJs and tTJs, respectively. White arrows are equivalent in length to the thickness of the cellular sheet. Bar, 10 μm.