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. 2005 Dec 19;171(6):1001–1012. doi: 10.1083/jcb.200508072

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Copyright © 2005, The Rockefeller University Press

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Figure 6. — PolyQ-mediated intranuclear htt accumulation in glial cells. (A) Immunofluorescent images of adenovirus-infected astrocytes that express GFP-htt containing a 23- (23Q) or 130- (130Q) glutamine repeat. Immunostaining with EM48 confirms that htt-130Q is located in the nucleus and forms nuclear aggregates. Nuclei are stained with Hoechst. (B) Proteasomal inhibition by ALLN (10 μg/ml) for 5 d significantly increases the formation of nuclear aggregates of htt-130Q, but not htt-23Q, in infected glial cells. (C) MTT assay of cultured glial cells infected by adenoviral-GFP (+GFP), htt-23Q (+23Q), or htt-130Q (+130Q) for 9 d. (D) MTT assay of astrocytes from R6/2 mice (HD) or littermate controls (WT) cultured for 6 or 8 wk. The data are presented as mean ± SEM (n = 3–4) and p values for 6- and 8-wk group comparisons (WT vs. HD) are 0.23 and 0.014, respectively. Bars, 5 μm.