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. 2005 Aug 29;170(5):757–767. doi: 10.1083/jcb.200504112

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Copyright © 2005, The Rockefeller University Press

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Figure 3. — Localization of AtRMR1 deletion mutants. (A) Expression of AtRMR1 deletion mutants. Protein extracts were obtained from protoplasts transformed with the indicated constructs and subjected to Western blot analysis using anti-HA and anti-AtRMR1 antibodies. Note that anti-AtRMR1 antibody does not recognize AtRMR1ΔCT. (B) Localization of AtRMR1 deletion mutants. Protoplasts transformed with the indicated constructs were fixed and stained with anti-HA or anti-myc antibodies. The green fluorescent signal of ST-GFP was directly observed from fixed protoplasts. Arrowheads indicate overlaps between the indicated proteins. Bars, 20 μm. (C) Quantification of the overlaps between AtRMR1 deletion mutants and ST-GFP or DIP-myc. The number of punctate stains of AtRMR1 deletion mutants and ST-GFP or DIP-myc that overlapped were counted to determine localization of AtRMR1 deletion mutants. More than 200 punctate stains of AtRMR1 deletion mutants were counted for each comparison in a triplicate experiment. Error bars represent SEM. WT, wild-type AtRMR1; ΔLU, AtRMR1ΔLU-HA; ΔCT, AtRMR1ΔCT-HA.