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. 2005 Jul 4;170(1):61–72. doi: 10.1083/jcb.200502070

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Copyright © 2005, The Rockefeller University Press

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Figure 3. — Effect of glucose deprivation on Akt1–JIP1/3 interaction or JIP1–JNK1/2 interaction. DU-145 cells were coinfected with Ad.HA-Akt1 and Ad.Flag-JIP1 (A) or Flag-tagged JIP3 (Ad.Flag-JIP3; B) at an MOI of 10. DU-145 cells were coinfected with Ad.Flag-JIP1 and Ad.His-JNK1 (C) or Ad.HA-JNK2 (D) at an MOI of 10. After 48 h of infection, cells were exposed to glucose-free medium for various times. (A and B) Cell lysates were immunoprecipitated with anti-HA antibody and were immunoblotted with anti-Flag or anti-HA antibody (top). The presence of Flag-JIP1, Flag-JIP3, phospho-Akt, or actin in the lysates was verified by immunoblotting (bottom). (C and D) Lysates were immunoprecipitated with anti-His/anti-HA antibody and were immunoblotted with anti-Flag or anti-His/anti-HA antibody (top). The presence of Flag-JIP1 in the lysates was verified by immunoblotting (bottom).