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. 2005 Jul 4;170(1):49–59. doi: 10.1083/jcb.200501067

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Copyright © 2005, The Rockefeller University Press

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Figure 6. — HIF1α plays a pro-survival role in B16 melanoma cells. (A) Immunofluorescence study to detect the presence of cleaved caspase-3 in B16 cells nonstimulated (NS) or stimulated for 24 with forskolin (FK), and nontreated (−St) or treated with staurosporin (+St) for 6 h. Bar, 40 μM. (B) Immunofluorescence analysis of B16 cells transfected with a nonrelevant siRNA (siRNA-Ctrol) or an siRNA to invalidate HIF1α. Cleaved caspase-3 was labeled with a secondary Texas red–conjugated antibody (red) and HIF1α protein was detected by an mAb and a secondary FITC-conjugated secondary one (green). Nuclei were labeled with bisbenzidine (blue). Bar, 20 μM.