Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Jul 4;170(1):141–151. doi: 10.1083/jcb.200412032

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — IRM reveals the areas of direct contact between migrating T cells and ICAM-1. (A) The DIC image compared with the IRM image and the ICAM-1 binding focal zone (red). The IRM image identifies the total area of the T cell in direct contact with ICAM-1 in the bilayer and demonstrates intermittent, close, and lack of attachments in lamellipodia, focal zone, and uropod, respectively. Double-sided arrow, closest attachment to the substrate; black arrow, the leading edge; white arrow, the uropod of the migrating T cell. Overlaying the ICAM-1 and IRM images shows that the area of uniform attachment colocalizes with the focal zone. (B) Quantification of the area in contact with the bilayer compared with the area of the focal zone (n = 10; ±SD). All images were taken with a 63× oil immersion objective. Bars, 10 μm.