Figure 1.

TCRζ chain expression is increased in SLAP−/− mice. (A) Western blot analysis of TCRα, CD3ɛ, and TCRζ expression in RIPA lysates of purified CD8+ thymocytes from WT and SLAP−/− mice. Data are representative of six independent experiments. (B) Quantitation of Western blots represented in A using quantitative luminescence. Data represent mean expression of TCRα, CD3ɛ, or TCRζ by SLAP−/− CD8+ thymocytes as compared with WT controls ± SEM. TCR–CD3 levels have been normalized to α-tubulin. (C) Surface or total TCRβ and CD3ɛ expression by either surface or intracellular FACS staining. Data are representative of six mice per genotype. (D) Quantitation of FACS staining shown in C. Data represent the mean fluorescence intensity (MFI) ± SEM (error bars) as compared with WT controls for six mice of each genotype.